Communication

Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters†

  1. Prof. Melanie A. Priestman1,
  2. Dr. Thomas A. Shell1,
  3. Dr. Liang Sun2,
  4. Prof. Hsien-Ming Lee3,
  5. Prof. David S. Lawrence1,*

Article first published online: 27 JUN 2012

DOI: 10.1002/anie.201202820

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 31, pages 7684–7687, July 27, 2012

Additional Information

How to Cite

Priestman, M. A., Shell, T. A., Sun, L., Lee, H.-M. and Lawrence, D. S. (2012), Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters . Angew. Chem. Int. Ed., 51: 7684–7687. doi: 10.1002/anie.201202820

Author Information

  1. 1

    Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)

  2. 2

    Pharmaron-Beijing BDA, Beijing, 100176 (P.R. China)

  3. 3

    Institute of Chemistry, Academia Sinica, Nankang Dist. Taipei, 115 (Taiwan)

*Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)

  1. †

    We thank the NIH (GM086217) for financial support.

Publication History

  1. Issue published online: 25 JUL 2012
  2. Article first published online: 27 JUN 2012
  3. Manuscript Received: 12 APR 2012

Funded by

  • NIH. Grant Number: GM086217

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Keywords:

  • caged probes;
  • cobalamines;
  • fluorescent probes;
  • fluorescence spectroscopy;
  • photolysis
Thumbnail image of graphical abstract

Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties that can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560 nm) have been synthesized and evaluated. This tri-color system is also applied to imaging in microwells and HeLa cells (see picture).

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