We thank the NIH (GM086217) for financial support.
Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters†
Article first published online: 27 JUN 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 31, pages 7684–7687, July 27, 2012
How to Cite
Priestman, M. A., Shell, T. A., Sun, L., Lee, H.-M. and Lawrence, D. S. (2012), Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters . Angew. Chem. Int. Ed., 51: 7684–7687. doi: 10.1002/anie.201202820
- Issue published online: 25 JUL 2012
- Article first published online: 27 JUN 2012
- Manuscript Received: 12 APR 2012
- NIH. Grant Number: GM086217
- caged probes;
- fluorescent probes;
- fluorescence spectroscopy;
Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties that can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560 nm) have been synthesized and evaluated. This tri-color system is also applied to imaging in microwells and HeLa cells (see picture).