Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters

Authors

  • Prof. Melanie A. Priestman,

    1. Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
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  • Dr. Thomas A. Shell,

    1. Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
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  • Dr. Liang Sun,

    1. Pharmaron-Beijing BDA, Beijing, 100176 (P.R. China)
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  • Prof. Hsien-Ming Lee,

    1. Institute of Chemistry, Academia Sinica, Nankang Dist. Taipei, 115 (Taiwan)
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  • Prof. David S. Lawrence

    Corresponding author
    1. Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
    • Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
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  • We thank the NIH (GM086217) for financial support.

Abstract

original image

Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties that can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560 nm) have been synthesized and evaluated. This tri-color system is also applied to imaging in microwells and HeLa cells (see picture).

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