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Keywords:

  • DNA polymerase;
  • magic-angle spinning;
  • NMR spectroscopy;
  • protein structures;
  • scalar transfers
Thumbnail image of graphical abstract

Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13Cα, and 13CO resonances and yields information about 1H–1H proximities.