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Backbone Assignment of Fully Protonated Solid Proteins by 1H Detection and Ultrafast Magic-Angle-Spinning NMR Spectroscopy


  • We acknowledge support from the Agence Nationale de la Recherche (ANR 08-BLAN-0035-01 and 10-BLAN-713-01), and from the Joint Research Activity within Research Infrastructures in the 7th Framework program of the EC (BioNMR, contract n. 261863), and from the Australian Research Council.


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Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13Cα, and 13CO resonances and yields information about 1H–1H proximities.

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