Combined Electrochemistry and Surface-Enhanced Infrared Absorption Spectroscopy of Gramicidin A Incorporated into Tethered Bilayer Lipid Membranes

Authors

  • Jacek Kozuch,

    1. Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany)
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  • Prof. Dr. Claudia Steinem,

    1. Institut für Organische und Biomolekulare Chemie, Universität Göttingen, Tammannstrasse 2, 37077 Göttingen (Germany)
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  • Prof. Dr. Peter Hildebrandt,

    Corresponding author
    1. Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany)
    • Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany)
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  • Dr. Diego Millo

    Corresponding author
    1. Biomolecular Spectroscopy/LaserLaB Amsterdam, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam (The Netherlands)
    • Biomolecular Spectroscopy/LaserLaB Amsterdam, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam (The Netherlands)
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  • We thank Lars J. C. Jeuken for helpful discussions. This work was supported by the DFG (Cluster of Excellence “UniCat”, P.H.; SFB 803, C.S.), the Alexander-von-Humboldt Foundation (D.M.), and the Netherlands Organisation for Scientific Research (NWO) (grant 722.011.003, D.M.). J.K. acknowledges a fellowship by the BIG-NSE. C.S. thanks the Brain Sustain Initiative of the University of Göttingen for granting a sabbatical leave and J. Gerber-Nolte for practical assistance.

Abstract

original image

Support from the support: Tethered bilayer lipid membranes containing the cation-channel-forming peptide gramicidin A were assembled on nanostructured Au films. The combination of surface-enhanced infrared absorption (SEIRA) and electrochemical impedance spectroscopy (EIS) was used for the in situ structural and functional characterization of gramicidin A in the same device.

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