We acknowledge financial support from the Department of Chemistry at North Carolina State University and the Beckman Foundation (Beckman Young Investigator award to A.D.).
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Communication
Hydrogen Peroxide Induced Activation of Gene Expression in Mammalian Cells using Boronate Estrone Derivatives†
Article first published online: 31 JUL 2012
DOI: 10.1002/anie.201203222
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 51, Issue 36, pages 9066–9070, September 3, 2012
Additional Information
How to Cite
Govan, J. M., McIver, A. L., Riggsbee, C. and Deiters, A. (2012), Hydrogen Peroxide Induced Activation of Gene Expression in Mammalian Cells using Boronate Estrone Derivatives . Angew. Chem. Int. Ed., 51: 9066–9070. doi: 10.1002/anie.201203222
- †
Publication History
- Issue published online: 28 AUG 2012
- Article first published online: 31 JUL 2012
- Manuscript Revised: 28 JUN 2012
- Manuscript Received: 26 APR 2012
Funded by
- Department of Chemistry at North Carolina State University
- Beckman Foundation
Keywords:
- biosensors;
- estrogen receptor;
- estrone;
- gene expression;
- hydrogen peroxide
Keeping the boron out of the ER: A genetic switch was engineered that activates gene expression in the presence of H2O2 (see scheme). The use of a boronate group on an estrone molecule allows for activation of gene expression through binding of the estrogen receptor only when the boron group is oxidized by H2O2. This sensor is highly sensitive and specific for H2O2.