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Solution Structure of a Parallel-Stranded Oligoisoguanine DNA Pentaplex Formed by d(T(iG)4T) in the Presence of Cs+ Ions

Authors

  • Dr. Mijeong Kang,

    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095-1569 (USA)
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  • Dr. Ben Heuberger,

    1. Department of Chemistry, University of California, Riverside, CA 92521 (USA)
    2. Current address: Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114 (USA)
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  • Dr. John C. Chaput,

    1. Department of Chemistry, University of California, Riverside, CA 92521 (USA)
    2. Current address: Department of Chemistry, Arizona State University (USA)
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  • Prof. Dr. Christopher Switzer,

    Corresponding author
    1. Department of Chemistry, University of California, Riverside, CA 92521 (USA)
    • Department of Chemistry, University of California, Riverside, CA 92521 (USA)
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  • Prof. Dr. Juli Feigon

    Corresponding author
    1. Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095-1569 (USA)
    • Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095-1569 (USA)
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  • Work in the group of J.F. is supported by the U.S. Department of Energy. Work in the group of C.S. is supported by NASA.

Abstract

original image

What a pentaplexing situation: Isoguanine (iG) is an isomer of guanine, where the positions of the C2 amino group and the C6 carboxy group are swapped. iG can self-assemble into pentads in the presence of alkali metal cations. Cs+ ions were found to stabilize a pentaplex of d(T(iG)4T). Solution NMR studies of this pentaplex in the presence of Cs+ ions (or Na+, K+, Rb+, or NH4+ cations) demonstrate its stability and structure (see picture).

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