Get access

Fibrous Nanostructures from the Self-Assembly of Designed Repeat Protein Modules

Authors

  • Dr. Jonathan J. Phillips,

    1. School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS (UK)
    Search for more papers by this author
  • Charlotte Millership,

    1. School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS (UK)
    Search for more papers by this author
  • Dr. Ewan R. G. Main

    Corresponding author
    1. School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS (UK)
    • School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS (UK)
    Search for more papers by this author

  • J.J.P and this work were supported by BBSRC Grant E005187/1 and QMUL. C.M is supported by a BBSRC studentship. We thank Dr. R. Rose, Dr. A. Lowe, and members of SBCS (QMUL) for critical reading of the manuscript and insightful discussions.

Abstract

original image

Single-protein-chain superhelical filaments are obtained from monomeric repeat proteins by controlling the chemistry and solvent exposure at their terminal interfaces. The assembly was achieved in aqueous solution, at neutral pH value, and at room temperature. The building block was a recombinantly engineered designed tetratricopeptide repeat protein. Directed head-to-tail self-assembly was driven by genetically encoded orthogonal native chemical ligation.

Ancillary