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Keywords:

  • DNA;
  • solid-phase synthesis;
  • primer extension;
  • protecting groups;
  • reversible terminator
Thumbnail image of graphical abstract

Extending both ways: A method for DNA-templated synthesis on solid support is described. Controlled, stepwise chain extension was demonstrated both in the direction favored by nature (3′-extension; see scheme) and in the direction typical for conventional DNA synthesizers (5′-extension).