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Template-Directed Synthesis in 3′- and 5′-Direction with Reversible Termination


  • This work was supported by the DFG (grant no. RI 1063/8-1 to C.R.) The authors thank Dr. E. Kervio and R. Haug for helpful discussions, and D. Göhringer, and T. Sabirov for skilled technical assistance.


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Extending both ways: A method for DNA-templated synthesis on solid support is described. Controlled, stepwise chain extension was demonstrated both in the direction favored by nature (3′-extension; see scheme) and in the direction typical for conventional DNA synthesizers (5′-extension).