A Combined Atomic Force Microscopy and Computational Approach for the Structural Elucidation of Breitfussin A and B: Highly Modified Halogenated Dipeptides from Thuiaria breitfussi


  • MabCent is a Centre at University of Tromsø and supported by the Research Council of Norway, Grant no 174885/130. Marbank is acknowledged for collection and taxonomy of Thuiaria breitfussi. Financial support from EU projects ARTIST (Contract No. 243421), HERODOT (Contract No. 214954), and the ERC advanced grant CEMAS, as well as Int.PhD.P.P. of the Foundation for Polish Science co-financed from E.R.D.F. “Grants for Innovation” and Research Council of Norway by grant no. 179568/V30 and 177558/V00, are gratefully acknowledged.


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Turning a new leaf: The first structures isolated from Thuiaria breitfussi, the breitfussins, are presented. This structural class consists of indole–oxazole–pyrrole units. Limited quantities prevented crystallization; therefore, the structures were solved using a novel combination of AFM, computer-aided structure elucidation (CASE), and DFT calculations. Visualization by AFM determined all the connection points of the cyclic systems and the other substituents.