“Self-Immolative” Spacer for Uncaging with Fluorescence Reporting

Authors

  • Dr. Raphaël Labruère,

    1. Institut Curie, Centre de Recherche, 26 rue d'Ulm, 75248 Paris (France)
    2. Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris (France)
    3. UMR 176, 26 rue d'Ulm, 75248 Paris (France)
    Search for more papers by this author
  • Dipl.-Chem. Ahmed Alouane,

    1. Institut Curie, Centre de Recherche, 26 rue d'Ulm, 75248 Paris (France)
    2. Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris (France)
    3. UMR 176, 26 rue d'Ulm, 75248 Paris (France)
    Search for more papers by this author
  • Dr. Thomas Le Saux,

    1. Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris (France)
    Search for more papers by this author
  • Dr. Isabelle Aujard,

    1. Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris (France)
    Search for more papers by this author
  • Dr. Philippe Pelupessy,

    1. Ecole Normale Supérieure, Département de Chimie, UMR CNRS-UPMC-ENS 7203 Laboratoire des Biomolécules, 24 rue Lhomond, 75231 Paris (France)
    Search for more papers by this author
  • Dr. Arnaud Gautier,

    1. Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris (France)
    Search for more papers by this author
  • Sylvie Dubruille,

    1. Institut Curie, Centre de Recherche, 26 rue d'Ulm, 75248 Paris (France)
    2. UMR 176, 26 rue d'Ulm, 75248 Paris (France)
    Search for more papers by this author
  • Dr. Frédéric Schmidt,

    Corresponding author
    1. Institut Curie, Centre de Recherche, 26 rue d'Ulm, 75248 Paris (France)
    2. UMR 176, 26 rue d'Ulm, 75248 Paris (France)
    • Institut Curie, Centre de Recherche, 26 rue d'Ulm, 75248 Paris (France)
    Search for more papers by this author
  • Prof. Dr. Ludovic Jullien

    Corresponding author
    1. Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris (France)
    • Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR, 24 rue Lhomond, 75231 Paris (France)
    Search for more papers by this author

  • This work has been supported by the ANR PCV 2008 (Proteophane) and ANR Blanc 2010 (Kituse).

Abstract

original image

Dual photoliberation: A caged, branched, self-immolative spacer (see scheme, gray box) was designed to rapidly and simultaneously release a desired compound (green) and a fluorophore (red) upon photoactivation. Careful kinetic analysis of the disassembly of the spacer shows that it occurs on the shortest time scale reported to date.

Ancillary