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Encapsulation of Biomacromolecules within Polymersomes by Electroporation

Authors

  • Dr. Linge Wang,

    1. The Krebs Institute (UK)
    2. The Centre for Membrane Interactions and Dynamics (UK)
    3. Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN (UK)
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    • These authors contributed equally.

  • Luca Chierico,

    1. The Krebs Institute (UK)
    2. The Centre for Membrane Interactions and Dynamics (UK)
    3. Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN (UK)
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    • These authors contributed equally.

  • Daniel Little,

    1. The Krebs Institute (UK)
    2. The Centre for Membrane Interactions and Dynamics (UK)
    3. Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN (UK)
    4. Sheffield Institute of Translational Neuroscience, The University of Sheffield, 385a Glossop Road, Sheffield, S10 2HQ (UK)
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    • These authors contributed equally.

  • Nisa Patikarnmonthon,

    1. The Krebs Institute (UK)
    2. The Centre for Membrane Interactions and Dynamics (UK)
    3. Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN (UK)
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    • These authors contributed equally.

  • Zhou Yang,

    1. Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN (UK)
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  • Prof. Dr. Mimoun Azzouz,

    1. Sheffield Institute of Translational Neuroscience, The University of Sheffield, 385a Glossop Road, Sheffield, S10 2HQ (UK)
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  • Dr. Jeppe Madsen,

    1. The Krebs Institute (UK)
    2. The Centre for Membrane Interactions and Dynamics (UK)
    3. Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN (UK)
    4. Department of Chemistry, The University of Sheffield, Brook Hill, Sheffield, S3 7HF (UK)
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  • Prof. Dr. Steven P. Armes,

    1. Department of Chemistry, The University of Sheffield, Brook Hill, Sheffield, S3 7HF (UK)
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  • Prof. Dr. Giuseppe Battaglia

    Corresponding author
    1. The Krebs Institute (UK)
    2. The Centre for Membrane Interactions and Dynamics (UK)
    3. Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN (UK)
    • The Krebs Institute (UK)
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Abstract

original image

Biological macromolecules can be encapsulated into preformed polymersomes by controlled temporary destabilization of the vesicle membrane. The morphology and the size of the polymersome are unchanged after electroporation, suggesting that the polymersome membrane is reformed. The surface charge of the biomacromolecules plays a key role for the electroporation process.

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