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Keywords:

  • enzyme catalysis;
  • kinases;
  • NMR spectroscopy;
  • proteins;
  • transition states
Thumbnail image of graphical abstract

Well-balanced: 19F NMR spectroscopy defined a trifluoromagnesate complex for protein kinase A (multicolored) with adenosine diphosphate (black), the MgF3 ion (green), and the SP20 peptide substrate (purple with dots). A sphere (cyan) centered on the MgF3 ion embraces all catalytic components and much of the SP20 substrate. The content of the sphere is uncharged conforming to the charge balance hypothesis.