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Charge-Balanced Metal Fluoride Complexes for Protein Kinase A with Adenosine Diphosphate and Substrate Peptide SP20

Authors

  • Dr. Yi Jin,

    1. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
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  • Dr. Matthew J. Cliff,

    1. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
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  • Dr. Nicola J. Baxter,

    1. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
    2. Manchester Institute of Biotechnology, Manchester, M1 7DN (UK)
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  • Dr. Hugh R. W. Dannatt,

    1. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
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  • Andrea M. Hounslow,

    1. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
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  • Dr. Matthew W. Bowler,

    1. European Molecular Biology Laboratory and Unit of Virus Host-Cell Interactions, UJF-EMBL-CNRS, UMI 3265, 6 rue Jules Horowitz, BP 181, 38042, Grenoble (France)
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  • Prof. Dr.  G. Michael Blackburn,

    Corresponding author
    1. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
    • Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
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  • Prof. Dr. Jonathan P. Waltho

    Corresponding author
    1. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
    2. Manchester Institute of Biotechnology, Manchester, M1 7DN (UK)
    • Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
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  • The expression construct for cAPK from mouse was provided by Dr. Peter Rellos, SGC, Oxford (UK). We thank the University of Sheffield for a Studentship (to Y.J.) and BBSRC for Grant No. I002146.

Abstract

original image

Well-balanced: 19F NMR spectroscopy defined a trifluoromagnesate complex for protein kinase A (multicolored) with adenosine diphosphate (black), the MgF3 ion (green), and the SP20 peptide substrate (purple with dots). A sphere (cyan) centered on the MgF3 ion embraces all catalytic components and much of the SP20 substrate. The content of the sphere is uncharged conforming to the charge balance hypothesis.

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