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Multiplexed In Situ Immunofluorescence Using Dynamic DNA Complexes

Authors

  • Dr. Ryan M. Schweller,

    1. Department of Bioengineering and Department of Chemistry, Rice University, 6100 Main Street, Houston, TX 77005 (USA)
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  • Jan Zimak,

    1. Department of Bioengineering and Department of Chemistry, Rice University, 6100 Main Street, Houston, TX 77005 (USA)
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  • Dr. Dzifa Y. Duose,

    1. Department of Bioengineering and Department of Chemistry, Rice University, 6100 Main Street, Houston, TX 77005 (USA)
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  • Dr. Amina A. Qutub,

    1. Department of Bioengineering and Department of Chemistry, Rice University, 6100 Main Street, Houston, TX 77005 (USA)
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  • Prof. Walter N. Hittelman,

    1. Department of Experimental Therapeutics, M. D. Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030 (USA)
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  • Dr. Michael R. Diehl

    Corresponding author
    1. Department of Bioengineering and Department of Chemistry, Rice University, 6100 Main Street, Houston, TX 77005 (USA)
    • Department of Bioengineering and Department of Chemistry, Rice University, 6100 Main Street, Houston, TX 77005 (USA)
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  • This work was supported in whole or in part by grants from the NIH (1R21A147912) and the Welch Foundation (C-1625). R.M.S. is supported by the Nanobiology Interdisciplinary Graduate Training Program of the W. M. Keck Center for Interdisciplinary Bioscience Training of the Gulf Coast Consortia (NIH grant no. T32 EB009379).

Abstract

original image

DNA-based erasers: The designed strand-displacement reactions between dynamic DNA probes (carrying fluorescent dyes (★) and quencher domains (•)) and DNA–antibody conjugates generate detachable immunofluoresence-reporting complexes that can be assembled (first image) and disassembled (second image) reversibly within fixed cells. With this system, different protein targets can be imaged sequentially within the same cells (third image).

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