We thank Dr. C. Neylon for providing an expression construct for sortase, Prof. K. Drickamer for providing a plasmid for expressing the ManBP variant, and Dr. T. Edwards and Dr. H. Jenkins for a sample of the pumilio protein. This work was supported by an EPSRC studentship for D.J.W. and also research grants EP/G043302/1, EP/I013083/1 and BB/G004145/1. W.B.T. thanks the Royal Society for a University Research Fellowship.
Efficient N-Terminal Labeling of Proteins by Use of Sortase†
Article first published online: 13 AUG 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 37, pages 9377–9380, September 10, 2012
How to Cite
Williamson, D. J., Fascione, M. A., Webb, M. E. and Turnbull, W. B. (2012), Efficient N-Terminal Labeling of Proteins by Use of Sortase . Angew. Chem. Int. Ed., 51: 9377–9380. doi: 10.1002/anie.201204538
- Issue published online: 5 SEP 2012
- Article first published online: 13 AUG 2012
- Manuscript Received: 11 JUN 2012
- Funded Access
- EPSRC. Grant Numbers: EP/G043302/1, EP/I013083/1, BB/G004145/1
“Sorting out” N-terminal labeling: The reversibility of transpeptidase reactions makes protein N-terminal labeling challenging. Depsipeptide substrates for sortase A release alcohol by-products, which are poor nucleophiles for the reverse reaction, during ligation. Proteins with an unhindered N-terminal glycine residue can be labeled efficiently with only a minimal excess of the labeling reagent (see scheme).