We thank Dr. Khiang Wee Lim, Director of the A*GA, A*STAR, Singapore for a grant for S.M. M. N. thanks A*STAR for financial support. E.-K. S. thanks Rain Fabricius-Brand for an introduction in interdisciplinary writing.
In Vitro Expressed GPCR Inserted in Polymersome Membranes for Ligand-Binding Studies†
Article first published online: 19 NOV 2012
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 2, pages 749–753, January 7, 2013
How to Cite
May, S., Andreasson-Ochsner, M., Fu, Z., Low , Y. X., Tan, D., de Hoog, H.-P. M., Ritz, S., Nallani, M. and Sinner, E.-K. (2013), In Vitro Expressed GPCR Inserted in Polymersome Membranes for Ligand-Binding Studies . Angew. Chem. Int. Ed., 52: 749–753. doi: 10.1002/anie.201204645
- Issue published online: 28 DEC 2012
- Article first published online: 19 NOV 2012
- Manuscript Revised: 2 AUG 2012
- Manuscript Received: 14 JUN 2012
- drug discovery;
- protein expression;
The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.