In Vitro Expressed GPCR Inserted in Polymersome Membranes for Ligand-Binding Studies

Authors

  • Sylvia May,

    1. Patterning and Fabrication, Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research, Research link 3, Singapore 117602 (Singapore)
    Search for more papers by this author
  • Mirjam Andreasson-Ochsner,

    1. Patterning and Fabrication, Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research, Research link 3, Singapore 117602 (Singapore)
    Search for more papers by this author
  • Zhikang Fu,

    1. Patterning and Fabrication, Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research, Research link 3, Singapore 117602 (Singapore)
    Search for more papers by this author
  • Ying Xiu Low ,

    1. Patterning and Fabrication, Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research, Research link 3, Singapore 117602 (Singapore)
    Search for more papers by this author
  • Dr. Darren Tan,

    1. Patterning and Fabrication, Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research, Research link 3, Singapore 117602 (Singapore)
    Search for more papers by this author
  • Dr. Hans-Peter M. de Hoog,

    1. Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553 (Singapore)
    Search for more papers by this author
  • Dr. Sandra Ritz,

    1. Physical Chemistry of Polymers, Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz (Germany)
    Search for more papers by this author
  • Dr. Madhavan Nallani,

    Corresponding author
    1. Patterning and Fabrication, Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research, Research link 3, Singapore 117602 (Singapore)
    • Patterning and Fabrication, Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research, Research link 3, Singapore 117602 (Singapore)
    Search for more papers by this author
  • Prof. Dr. Eva-Kathrin Sinner

    Corresponding author
    1. University of Natural Resources and Life Science, BOKU, Vienna, Department of NanoBiotechnology, Muthgasse 11, 1190 Vienna (Austria)
    • University of Natural Resources and Life Science, BOKU, Vienna, Department of NanoBiotechnology, Muthgasse 11, 1190 Vienna (Austria)
    Search for more papers by this author

  • We thank Dr. Khiang Wee Lim, Director of the A*GA, A*STAR, Singapore for a grant for S.M. M. N. thanks A*STAR for financial support. E.-K. S. thanks Rain Fabricius-Brand for an introduction in interdisciplinary writing.

Abstract

original image

The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.

Ancillary