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Keywords:

  • 5-hydroxymethylcytosine;
  • DNA;
  • enzymes;
  • oxidation;
  • sequencing
Thumbnail image of graphical abstract

Sodium bisulfite promotes both the deformylative deamination of 5-formylcytosine (5fC) and the decarboxylative deamination of 5-carboxylcytosine (5caC; see picture). By coupling this bisulfite chemistry with selective oxidations of individual DNA bases, new methods allow, for the first time, the sequencing of 5-hydroxymethylcytosine (5hmC) with single-base-pair resolution.