We thank the Helmholtz Drug Research Initiative for funding (P.S.) and the program “epigenetics@dkfz”.
Sequencing the Sixth Base (5-Hydroxymethylcytosine): Selective DNA Oxidation Enables Base-Pair Resolution†
Article first published online: 26 SEP 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 43, pages 10704–10707, October 22, 2012
How to Cite
Schüler, P. and Miller, A. K. (2012), Sequencing the Sixth Base (5-Hydroxymethylcytosine): Selective DNA Oxidation Enables Base-Pair Resolution . Angew. Chem. Int. Ed., 51: 10704–10707. doi: 10.1002/anie.201204768
- Issue published online: 17 OCT 2012
- Article first published online: 26 SEP 2012
- Manuscript Received: 18 JUN 2012
Sodium bisulfite promotes both the deformylative deamination of 5-formylcytosine (5fC) and the decarboxylative deamination of 5-carboxylcytosine (5caC; see picture). By coupling this bisulfite chemistry with selective oxidations of individual DNA bases, new methods allow, for the first time, the sequencing of 5-hydroxymethylcytosine (5hmC) with single-base-pair resolution.