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Keywords:

  • DNA;
  • solid-phase synthesis;
  • primer extension;
  • protecting groups;
  • reversible terminator
Thumbnail image of graphical abstract

Simultaneous chemical primer extension in both 3′- and 5′-direction is reported by C. Richert et al. in their Communication on page 8299 ff. The use of magnetic beads as a solid support and azidomethyloxycarbonyl moieties as protecting groups provided control over the extension steps directed by a DNA sequence. The method combines features of replication and of automated DNA synthesis.