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2′-Fluoro RNA Shows Increased Watson–Crick H-Bonding Strength and Stacking Relative to RNA: Evidence from NMR and Thermodynamic Data

Authors


  • Supported by NIH grants R01 GM055237 (M.E.) and R01 GM071461 (E.R.). We thank Dr. Markus Voehler and Dr. Min-Kyu Cho for help with NMR acquisition, Raquel Rozner and Ryan Merrihue for assistance with UV melting experiments, and Prof. Nicholas Reiter for helpful discussions.

Abstract

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The way it is: Incorporation of 2′-deoxy-2′-fluororibonucleotides (2′-F RNA) into RNA oligomers leads to a marked increase in duplex stability. Using NMR spectroscopy (see picture) with native (red circles) and 2′-F modified (black squares) RNA duplexes, and UV melting and osmotic stress experiments the higher stability of 2′-F RNA relative to RNA is shown to be the result of both increased Watson–Crick H-bonding strength and favorable base stacking.

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