Get access

2′-Fluoro RNA Shows Increased Watson–Crick H-Bonding Strength and Stacking Relative to RNA: Evidence from NMR and Thermodynamic Data


  • Supported by NIH grants R01 GM055237 (M.E.) and R01 GM071461 (E.R.). We thank Dr. Markus Voehler and Dr. Min-Kyu Cho for help with NMR acquisition, Raquel Rozner and Ryan Merrihue for assistance with UV melting experiments, and Prof. Nicholas Reiter for helpful discussions.


original image

The way it is: Incorporation of 2′-deoxy-2′-fluororibonucleotides (2′-F RNA) into RNA oligomers leads to a marked increase in duplex stability. Using NMR spectroscopy (see picture) with native (red circles) and 2′-F modified (black squares) RNA duplexes, and UV melting and osmotic stress experiments the higher stability of 2′-F RNA relative to RNA is shown to be the result of both increased Watson–Crick H-bonding strength and favorable base stacking.