These authors contributed equally to this work.
Epidithiol Formation by an Unprecedented Twin Carbon–Sulfur Lyase in the Gliotoxin Pathway†
Article first published online: 31 AUG 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 40, pages 10064–10068, October 1, 2012
How to Cite
Scharf, D. H., Chankhamjon, P., Scherlach, K., Heinekamp, T., Roth, M., Brakhage, A. A. and Hertweck, C. (2012), Epidithiol Formation by an Unprecedented Twin Carbon–Sulfur Lyase in the Gliotoxin Pathway . Angew. Chem. Int. Ed., 51: 10064–10068. doi: 10.1002/anie.201205041
We thank A. Perner for MS measurements, S. Fricke and C. Schult for technical assistance, P. Hortschansky for artwork, and C. Heiden and P. Berthel for performing fermentations. This work was supported by the “Pakt für Forschung und Innovation” of the Free State of Thuringia and the BMBF, and the International Leibniz Research School for Biomolecular and Microbial Interactions (ILRS), as part of the excellence graduate school Jena School for Microbial Communication (JSMC).
- Issue published online: 26 SEP 2012
- Article first published online: 31 AUG 2012
- Manuscript Received: 27 JUN 2012
- Free State of Thuringia
- International Leibniz Research School for Biomolecular and Microbial Interactions (ILRS)
- Aspergillus fumigatus;
- organosulfur compounds
Two in one go: The elucidation of a key step in the biosynthesis of gliotoxin, the infamous virulence factor of the human pathogen Aspergillus fumigatus, provides insight into the formation of an epidithiol. Isolation of a bis(cysteine) S-conjugate from a ΔgliI mutant and in vitro studies show that GliI concomitantly cleaves two CS bonds, along with the formation of ammonia and pyruvate (see scheme).