Communication

Signal Amplification by Glyco-qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology†

  1. Dr. Seok Joon Kwon1,‡,
  2. Prof. Kyung Bok Lee2,‡,
  3. Kemal Solakyildirim1,‡,
  4. Sayaka Masuko1,
  5. Mellisa Ly1,
  6. Dr. Fuming Zhang1,
  7. Dr. Lingyun Li1,
  8. Prof. Jonathan S. Dordick1,
  9. Prof. Robert J. Linhardt1,*

Article first published online: 16 OCT 2012

DOI: 10.1002/anie.201205112

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 47, pages 11800–11804, November 19, 2012

Additional Information

How to Cite

Kwon, S. J., Lee, K. B., Solakyildirim, K., Masuko, S., Ly, M., Zhang, F., Li, L., Dordick, J. S. and Linhardt, R. J. (2012), Signal Amplification by Glyco-qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology . Angew. Chem. Int. Ed., 51: 11800–11804. doi: 10.1002/anie.201205112

Author Information

  1. 1

    Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)

  2. 2

    Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-718 (Republic of Korea)

  1. ‡

    These authors contributed equally to this work.

*Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)

  1. †

    The authors thank Leyla Gasimli for critical reading of this manuscript, the National Institutes of Health for support in the form of grants no. GM38060, HL101721, and ES020903, and New York Stem Cell Science in the form of grant no. C024334.

  2. ‡

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 14 NOV 2012
  2. Article first published online: 16 OCT 2012
  3. Manuscript Revised: 4 SEP 2012
  4. Manuscript Received: 29 JUN 2012

Funded by

  • National Institutes of Health. Grant Numbers: GM38060, HL101721, ES020903
  • New York Stem Cell Science. Grant Number: C024334

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Keywords:

  • carbohydrates;
  • DNA;
  • glycoconjugates;
  • signal amplification;
  • ultrasensitive detection
Thumbnail image of graphical abstract

Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery.

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