These authors contributed equally to this work.
Signal Amplification by Glyco-qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology†
Article first published online: 16 OCT 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 47, pages 11800–11804, November 19, 2012
How to Cite
Kwon, S. J., Lee, K. B., Solakyildirim, K., Masuko, S., Ly, M., Zhang, F., Li, L., Dordick, J. S. and Linhardt, R. J. (2012), Signal Amplification by Glyco-qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology . Angew. Chem. Int. Ed., 51: 11800–11804. doi: 10.1002/anie.201205112
The authors thank Leyla Gasimli for critical reading of this manuscript, the National Institutes of Health for support in the form of grants no. GM38060, HL101721, and ES020903, and New York Stem Cell Science in the form of grant no. C024334.
- Issue published online: 14 NOV 2012
- Article first published online: 16 OCT 2012
- Manuscript Revised: 4 SEP 2012
- Manuscript Received: 29 JUN 2012
- National Institutes of Health. Grant Numbers: GM38060, HL101721, ES020903
- New York Stem Cell Science. Grant Number: C024334
- signal amplification;
- ultrasensitive detection
Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery.