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Signal Amplification by Glyco-qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology

Authors

  • Dr. Seok Joon Kwon,

    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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    • These authors contributed equally to this work.

  • Prof. Kyung Bok Lee,

    1. Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-718 (Republic of Korea)
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    • These authors contributed equally to this work.

  • Kemal Solakyildirim,

    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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    • These authors contributed equally to this work.

  • Sayaka Masuko,

    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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  • Mellisa Ly,

    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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  • Dr. Fuming Zhang,

    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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  • Dr. Lingyun Li,

    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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  • Prof. Jonathan S. Dordick,

    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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  • Prof. Robert J. Linhardt

    Corresponding author
    1. Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
    • Department of Chemistry and Chemical Biology, Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180 (USA)
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  • The authors thank Leyla Gasimli for critical reading of this manuscript, the National Institutes of Health for support in the form of grants no. GM38060, HL101721, and ES020903, and New York Stem Cell Science in the form of grant no. C024334.

Abstract

original image

Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery.

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