This work was supported by the NIH grant CA 091901 to J.R.
Cooperation of Two Bifunctional Enzymes in the Biosynthesis and Attachment of Deoxysugars of the Antitumor Antibiotic Mithramycin†
Article first published online: 20 SEP 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 42, pages 10638–10642, October 15, 2012
How to Cite
Wang, G., Pahari, P., Kharel, M. K., Chen, J., Zhu, H., Van Lanen, S. G. and Rohr, J. (2012), Cooperation of Two Bifunctional Enzymes in the Biosynthesis and Attachment of Deoxysugars of the Antitumor Antibiotic Mithramycin . Angew. Chem. Int. Ed., 51: 10638–10642. doi: 10.1002/anie.201205414
- Issue published online: 10 OCT 2012
- Article first published online: 20 SEP 2012
- Manuscript Received: 9 JUL 2012
- NIH. Grant Number: CA 091901
- bifunctional enzymes;
Two bifunctional enzymes cooperate in the assembly and the positioning of two sugars, D-olivose and D-mycarose, of the anticancer antibiotic mithramycin. MtmC finishes the biosynthesis of both sugar building blocks depending on which MtmGIV activity is supported. MtmGIV transfers these two sugars onto two structurally distinct acceptor substrates. The dual function of these enzymes explains two essential but previously unidentified activities.