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Heterologous Expression and Manipulation of Three Tetracycline Biosynthetic Pathways

Authors

  • Peng Wang,

    1. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095 (USA)
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  • Dr. Woncheol Kim,

    1. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095 (USA)
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  • Dr. Lauren B. Pickens,

    1. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095 (USA)
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  • Xue Gao,

    1. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095 (USA)
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  • Prof. Yi Tang

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095 (USA)
    • Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095 (USA)
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  • This research was supported by an NSF CBET grant and a David and Lucile Packard Foundation Fellowship to Y.T.

Abstract

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A very accommodating host: Three tetracycline biosynthetic pathways were overexpressed and manipulated in the heterologous host Streptomyces lividans K4-114. Through the inactivation of various genes and characterization of the resulting biosynthetic intermediates, new tetracycline-modifying enzymes were identified (see scheme).

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