We acknowledge Dr. Morten Munch Nielsen for his contribution with the initial cloning and expression of starch synthase I, and Prof. Monica M. Palcic for her guidance in all of the enzymology aspects of this work.
A Simple Synthesis of Sugar Nucleoside Diphosphates by Chemical Coupling in Water†
Article first published online: 12 OCT 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 46, pages 11531–11534, November 12, 2012
How to Cite
Tanaka, H., Yoshimura, Y., Jørgensen, M. R., Cuesta-Seijo, J. A. and Hindsgaul, O. (2012), A Simple Synthesis of Sugar Nucleoside Diphosphates by Chemical Coupling in Water . Angew. Chem. Int. Ed., 51: 11531–11534. doi: 10.1002/anie.201205433
- Issue published online: 7 NOV 2012
- Article first published online: 12 OCT 2012
- Manuscript Revised: 1 SEP 2012
- Manuscript Received: 10 JUL 2012
- pyrophosphate formation;
- sugar nucleotide synthesis;
- synthetic methods
Sugar nucleotides made easy: The new reagent “ImIm”, which is formed in situ in water, is shown to activate nucleoside 5′-phosphates to their imidazolides, these can subsequently couple with sugar-1-phosphates; the whole procedure takes place in water. This truly simple method yields a crude product mixture that can be used directly as a source of donors for glycosyltransferase-mediated oligsaccharide synthesis. In the scheme, B stands for the nucleobases U, A, or G.