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Keywords:

  • glycosyltransferases;
  • oligosaccharides;
  • pyrophosphate formation;
  • sugar nucleotide synthesis;
  • synthetic methods
Thumbnail image of graphical abstract

Sugar nucleotides made easy: The new reagent “ImIm”, which is formed in situ in water, is shown to activate nucleoside 5′-phosphates to their imidazolides, these can subsequently couple with sugar-1-phosphates; the whole procedure takes place in water. This truly simple method yields a crude product mixture that can be used directly as a source of donors for glycosyltransferase-mediated oligsaccharide synthesis. In the scheme, B stands for the nucleobases U, A, or G.