These authors contributed equally to the work.
β2-Adrenergic Receptor Solutions for Structural Biology Analyzed with Microscale NMR Diffusion Measurements†
Article first published online: 24 OCT 2012
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 1, pages 331–335, January 2, 2013
How to Cite
Horst, R., Stanczak, P., Stevens, R. C. and Wüthrich, K. (2013), β2-Adrenergic Receptor Solutions for Structural Biology Analyzed with Microscale NMR Diffusion Measurements. Angew. Chem. Int. Ed., 52: 331–335. doi: 10.1002/anie.201205474
This work was supported by the NIH Roadmap initiative grant P50 GM073197 for technology development and the PSI:Biology grant U54 GM094618. for GPCR biology studies. The authors thank Jeffrey J. Liu for a critical reading of the manuscript.
- Issue published online: 20 DEC 2012
- Article first published online: 24 OCT 2012
- Manuscript Received: 3 JUL 2012
- NIH. Grant Numbers: P50 GM073197, U54 GM094618
- diffusion coefficients;
- membrane proteins;
- NMR spectroscopy;
- structure elucidation
Microcoil NMR measurements were performed to determine the final composition of solutions of the β2-adrenergic receptor (β2AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β2AR (see picture). Standards are established for the reproducible preparation of G-protein-coupled receptor solutions for crystallization trials and solution NMR studies.