DNA Detection Based on Fluorogenic Nanospheres

Authors

  • Xin Shu,

    1. Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 (China)
    Search for more papers by this author
  • Yonghui Liu,

    1. Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 (China)
    Search for more papers by this author
  • Prof. Dr. Jin Zhu

    Corresponding author
    1. Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 (China)
    • Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093 (China)
    Search for more papers by this author

  • J.Z. gratefully acknowledges support from the National Natural Science Foundation of China (21274058) and the National Basic Research Program of China (2013CB922101, 2011CB935801).

Abstract

original image

High and dry fluorescence: Fluorogenic nanospheres (green, see scheme) are conjugated to DNA (pink) and used to detect target DNA (aqua). Upon addition of N-butylmorpholine (droplet), the nanospheres dissolve, releasing fluorophores, and intense blue fluorescence is emitted at the site of DNA hybridization. The separation of DNA hybridization and signal amplification gives high sensitivity (100 zmol) and selectivity.

Ancillary