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Keywords:

  • DNA detection;
  • fluorescent probes;
  • fluorogenic nanospheres;
  • nanoparticles;
  • signal amplification
Thumbnail image of graphical abstract

High and dry fluorescence: Fluorogenic nanospheres (green, see scheme) are conjugated to DNA (pink) and used to detect target DNA (aqua). Upon addition of N-butylmorpholine (droplet), the nanospheres dissolve, releasing fluorophores, and intense blue fluorescence is emitted at the site of DNA hybridization. The separation of DNA hybridization and signal amplification gives high sensitivity (100 zmol) and selectivity.