This work was supported by the Netherlands Organization for Scientific Research (NWO, Rubicon Fellowship, A.J.B.), the Medical Research Council, and Oxford Nanopore Technologies.
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Communication
Continuous Stochastic Detection of Amino Acid Enantiomers with a Protein Nanopore†
Article first published online: 29 AUG 2012
DOI: 10.1002/anie.201205687
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 51, Issue 38, pages 9606–9609, September 17, 2012
Additional Information
How to Cite
Boersma, A. J. and Bayley, H. (2012), Continuous Stochastic Detection of Amino Acid Enantiomers with a Protein Nanopore . Angew. Chem. Int. Ed., 51: 9606–9609. doi: 10.1002/anie.201205687
- †
Publication History
- Issue published online: 13 SEP 2012
- Article first published online: 29 AUG 2012
- Manuscript Received: 17 JUL 2012
Funded by
- Netherlands Organization for Scientific Research
- Medical Research Council
- Oxford Nanopore Technologies
Keywords:
- amino acids;
- biosensors;
- chirality;
- copper;
- membrane proteins
A stochastic sensing method: Discrimination between enantiomeric amino acids is achieved when the amino acids bind to a CuII complex within a protein nanopore sensor, which provides a chiral environment. The potential of the method is demonstrated by real-time observation of the increase in enantiomeric excess during an enzymatic kinetic resolution.