Get access

Changing the Enzyme Reaction Rate in Magnetic Nanosuspensions by a Non-Heating Magnetic Field

Authors

  • Prof. Dr. Natalia L. Klyachko,

    1. Department of Pharmaceutical Sciences and Center for Drug Delivery and Nanomedicine, University of Nebraska Medical Center, 96025 Nebraska Medical Center, Omaha, NE 68198 (USA)
    2. Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991 (Russia)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. Marina Sokolsky-Papkov,

    1. Department of Pharmaceutical Sciences and Center for Drug Delivery and Nanomedicine, University of Nebraska Medical Center, 96025 Nebraska Medical Center, Omaha, NE 68198 (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Nikorn Pothayee,

    1. Macromolecules and Interfaces Institute, Department of Chemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 (USA)
    Search for more papers by this author
  • Maria V. Efremova,

    1. Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991 (Russia)
    Search for more papers by this author
  • Dr. Dmitry A. Gulin,

    1. Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991 (Russia)
    Search for more papers by this author
  • Nipon Pothayee,

  • Artem A. Kuznetsov,

    1. Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991 (Russia)
    Search for more papers by this author
  • Dr. Alexander G. Majouga,

    1. Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991 (Russia)
    Search for more papers by this author
  • Prof. Judy S. Riffle,

    1. Macromolecules and Interfaces Institute, Department of Chemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 (USA)
    Search for more papers by this author
  • Prof. Dr. Yuri I. Golovin,

    1. Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991 (Russia)
    2. Nanocenter of Tambov State University, Tambov State University, Tambov, 392000 (Russia)
    Search for more papers by this author
  • Prof. Dr. Alexander V. Kabanov

    Corresponding author
    1. Department of Pharmaceutical Sciences and Center for Drug Delivery and Nanomedicine, University of Nebraska Medical Center, 96025 Nebraska Medical Center, Omaha, NE 68198 (USA)
    2. Laboratory for Chemical Design of Bionanomaterials, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991 (Russia)
    • Department of Pharmaceutical Sciences and Center for Drug Delivery and Nanomedicine, University of Nebraska Medical Center, 96025 Nebraska Medical Center, Omaha, NE 68198 (USA)
    Search for more papers by this author

  • The authors thank U.S. National Institutes of Health, RR021937, Russian Ministry of Science and Education, 11.G34.31.0004 (both to A.V.K.), and the U.S. National Science Foundation Materials World Network program, DMR-0805179 and the U.S. National Science Foundation contract DMR 0909065 (to J.S.R.). The authors are grateful to T. Bargar (UNMC) for carrying out TEM experiments, Dr. I. Khutsishvili (UNMC) for CD measurements, and Nazar Filonov (COBRE Nanomaterials Core Characterization) for ICP-MS measurements.

Abstract

original image

Deactivation and structural deformation of enzymes immobilized on magnetic nanoparticles (MNPs) are observed after exposure to magnetic fields. These changes are due to realignment of MNPs in response to the AC magnetic field, which leads to stresses in MNP-linked polymer chains. Such stresses translated to an enzyme molecule immobilized on a MNP aggregate induce deformations and irreversible (or long lasting) conformational changes.

Get access to the full text of this article

Ancillary