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A Switchable Two-Photon Membrane Tracer Capable of Imaging Membrane-Associated Protein Tyrosine Phosphatase Activities


  • Funding was provided by the Agency for Science, Technology and Research (R-143-000-391-305) and the Ministry of Education (R-143-000-394-112). We also acknowledge the generous donation of Drosophila tissues from Dr. Kah-Leong Lim and Dr. Chengwu Zhang (NUS).


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When I look into your cells: A two-photon dye (Flu7) was developed (see scheme), which strongly fluoresces only upon selective binding to the plasma membrane of mammalian cells. Upon addition of a UV- and phosphatase-controlled quencher (Q12), the system exhibits ON/OFF/ON fluorescence switching and can be used to image membrane-associated receptor-like protein tyrosine phosphatase (RPTP) activity.

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