These authors contributed equally to this work.
Ligand- and pH-Induced Conformational Changes of RNA Domain Helix 69 Revealed by 2-Aminopurine Fluorescence†
Article first published online: 24 OCT 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 48, pages 12095–12098, November 26, 2012
How to Cite
Sakakibara, Y., Abeysirigunawardena, S. C., Duc, A.-C. E., Dremann, D. N. and Chow, C. S. (2012), Ligand- and pH-Induced Conformational Changes of RNA Domain Helix 69 Revealed by 2-Aminopurine Fluorescence . Angew. Chem. Int. Ed., 51: 12095–12098. doi: 10.1002/anie.201206000
We thank D. Rueda for providing access to the fluorometer. This project was supported by the NIH (GM087596).
- Issue published online: 21 NOV 2012
- Article first published online: 24 OCT 2012
- Manuscript Received: 26 JUL 2012
- NIH. Grant Number: GM087596
- fluorescent probes;
- helix 69;
Loop conformation: The loop of the RNA domain helix 69 (H69) was modified with the fluorescent analogue 2-aminopurine (2AP), thus showing different conformational states under various conditions. The application of this model RNA reveals the unique impact of aminoglycoside neomycin, which differs from the effects of structurally related compounds paromomycin and gentamicin, on the H69 loop conformation in solution (see picture).