The authors acknowledge financial support from the EU (ERC starting grant NUCLEOPOLY and collaborative project ECCell), the Netherlands Organization for Scientific Research (NWO-Vici), and the Zernike Institute for Advanced Materials. The help of Andrew Musser and Alessio Marcozzi is appreciated very much by the authors.
1521-3773/asset/2002_left.gif?v=1&s=ac6b0d94a94d7ce7a210002b8096b42feffc0bcf)
1521-3773/asset/olbannercenter.gif?v=1&s=c083e1920cd41ed129901c116018eab93b5ad3c4)
1521-3773/asset/2002_right.gif?v=1&s=451042aa3415ae3ad0729984d26dee1866aca82e)
Communication
You have full text access to this OnlineOpen article
Modular Assembly of a Pd Catalyst within a DNA Scaffold for the Amplified Colorimetric and Fluorimetric Detection of Nucleic Acids†
Article first published online: 17 OCT 2012
DOI: 10.1002/anie.201206006
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
Angewandte Chemie International Edition
Volume 51, Issue 47, pages 11894–11898, November 19, 2012
Additional Information
How to Cite
Prusty, D. K., Kwak, M., Wildeman, J. and Herrmann, A. (2012), Modular Assembly of a Pd Catalyst within a DNA Scaffold for the Amplified Colorimetric and Fluorimetric Detection of Nucleic Acids . Angew. Chem. Int. Ed., 51: 11894–11898. doi: 10.1002/anie.201206006
- †
Publication History
- Issue published online: 14 NOV 2012
- Article first published online: 17 OCT 2012
- Manuscript Received: 26 JUL 2012
Funded by
- Funded Access
Keywords:
- bodipy;
- DNA catalyst;
- DNA sensing;
- fluorogenic reaction;
- signal amplification
Phosphine ligands were covalently tethered to short oligonucleotide strands such that active palladium complexes formed in the presence of a specific nucleic acid target. This catalytic center on the double-stranded DNA then converted water-soluble iodo-BODIPY dyes, present in excess, into highly emissive deiodinated reporters (see picture). This approach is suitable for the rapid colorimetric or fluorimetric detection of nucleic acid targets with a lower detection limit of just 10 pM.