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Keywords:

  • cell adhesion;
  • cell culture;
  • cell density gradient;
  • interface tissue engineering
Thumbnail image of graphical abstract

Making the grad(ient): A gradient in cell density was generated on a substrate that was inserted into a homogeneous suspension of cells at a specific tilt angle by taking advantage of the gradual change in the number of cells available for sedimentation (see scheme). Reverse gradients were also fabricated on the same substrate using multiple sedimentation procedures.