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Generation of Controllable Gradients in Cell Density

Authors

  • Wenying Liu,

    1. School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 (USA)
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  • Yu Zhang,

    1. The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332 (USA)
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  • Prof. Stavros Thomopoulos,

    1. Department of Orthopaedic Surgery, Washington University, School of Medicine, St. Louis, MO 63110 (USA)
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  • Prof. Younan Xia

    Corresponding author
    1. The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, School of Chemistry and Biochemistry and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 (USA)
    • The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, School of Chemistry and Biochemistry and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 (USA)

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  • This work was supported in part by a grant from the NIH (1R01 AR060820) and startup funds from the Georgia Institute of Technology.

Abstract

original image

Making the grad(ient): A gradient in cell density was generated on a substrate that was inserted into a homogeneous suspension of cells at a specific tilt angle by taking advantage of the gradual change in the number of cells available for sedimentation (see scheme). Reverse gradients were also fabricated on the same substrate using multiple sedimentation procedures.

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