At Last: Erythropoietin as a Single Glycoform

Authors

  • Dr. Ping Wang,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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    • These authors contributed equally to this work.

  • Dr. Suwei Dong,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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    • These authors contributed equally to this work.

  • Dr. John A. Brailsford,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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  • Dr. Karthik Iyer,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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  • Dr. Steven D. Townsend,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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  • Dr. Qiang Zhang,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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  • Dr. Ronald C. Hendrickson,

    1. Department of Pharmacology and Chemistry, Sloan-Kettering Institute for Cancer Research (USA)
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  • Dr. JaeHung Shieh,

    1. Cell Biology Program, Sloan-Kettering Institute for Cancer Research (USA)
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  • Dr. Malcolm A. S. Moore,

    1. Cell Biology Program, Sloan-Kettering Institute for Cancer Research (USA)
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  • Prof. Samuel J. Danishefsky

    Corresponding author
    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
    2. Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027 (USA)
    • Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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  • This research was supported by NIH grants CA28824 and HL025848 (S.J.D.). S.D.T. is grateful to Weill Cornell Medical School for an NIH postdoctoral fellowship.

Abstract

original image

Wild thing: The first total synthesis of wild-type erythropoietin glycoprotein (see picture) has been accomplished. The erythropoietic activity of the synthetic folded protein has been demonstrated.

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