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Observation of Multiphoton-Induced Fluorescence from Graphene Oxide Nanoparticles and Applications in In Vivo Functional Bioimaging

Authors

  • Dr. Jun Qian,

    1. Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University (China)
    2. JORCEP (Joint Research Center of Photonics of the Royal Institute of Technology (Sweden), Lund University (Sweden), and Zhejiang University (ZJU)), Hangzhou, Zhejiang, 310058 (China)
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    • These authors contributed equally to this work.

  • Dan Wang,

    1. Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University (China)
    2. JORCEP (Joint Research Center of Photonics of the Royal Institute of Technology (Sweden), Lund University (Sweden), and Zhejiang University (ZJU)), Hangzhou, Zhejiang, 310058 (China)
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    • These authors contributed equally to this work.

  • Fu-Hong Cai,

    1. Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University (China)
    2. JORCEP (Joint Research Center of Photonics of the Royal Institute of Technology (Sweden), Lund University (Sweden), and Zhejiang University (ZJU)), Hangzhou, Zhejiang, 310058 (China)
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  • Dr. Wang Xi,

    1. Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, School of Medicine, Zhejiang University, Hangzhou, Zhejiang (China)
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  • Li Peng,

    1. ZJU-SCNU Joint Research Center of Photonics, South China Normal University (SCNU), Guangzhou, 510006 (China)
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  • Zhen-Feng Zhu,

    1. Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University (China)
    2. JORCEP (Joint Research Center of Photonics of the Royal Institute of Technology (Sweden), Lund University (Sweden), and Zhejiang University (ZJU)), Hangzhou, Zhejiang, 310058 (China)
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  • Dr. Hao He,

    1. Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Key Laboratory of Opto-Electronics Information and Technical Science, Ministry of Education, Tianjin University, Tianjin 300072 (China)
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  • Prof. Ming-Lie Hu,

    1. Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Key Laboratory of Opto-Electronics Information and Technical Science, Ministry of Education, Tianjin University, Tianjin 300072 (China)
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  • Prof. Sailing He

    Corresponding author
    1. Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University (China)
    2. JORCEP (Joint Research Center of Photonics of the Royal Institute of Technology (Sweden), Lund University (Sweden), and Zhejiang University (ZJU)), Hangzhou, Zhejiang, 310058 (China)
    3. ZJU-SCNU Joint Research Center of Photonics, South China Normal University (SCNU), Guangzhou, 510006 (China)
    • Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University (China)
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  • This work was partially supported by the Science and Technology Department of Zhejiang Province, the National Basic Research Program (973) of China (2011CB503700), the Special Financial Grant from the China Postdoctoral Science Foundation (No. 201104741), the National Natural Science Foundation of China (61275190, 61008052, 61178062, and 60990322), and the Fundamental Research Funds for the Central Universities. J.Q. is grateful to Dr. S. S. Liu for help with two-photon laser scanning confocal microscopy. We also express our deepest gratitude towards Prof. Z. P. Xu’s group for their help in the cell proliferation assay.

Abstract

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Lightening organelles: A femtosecond laser can excite multiphoton-induced luminescence of graphene oxide nanoparticles. The flow, distributions, and clearance of intravenously injected GO-PEG nanoparticles in the blood vessel of mice could be observed clearly by two-photon imaging. The 3D distribution of microinjected GO-PEG nanoparticles in a mice brain could also be reconstructed with two-photon microscopy.

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