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Quantum-Dot Fluorescence Lifetime Engineering with DNA Origami Constructs

Authors

  • Dr. Seung Hyeon Ko,

    1. Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA)
    2. Maryland Nanocenter, University of Maryland, College Park, MD 20742 (USA)
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    • These authors contributed equally to this work.

  • Kan Du,

    1. Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA)
    2. Maryland Nanocenter, University of Maryland, College Park, MD 20742 (USA)
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    • These authors contributed equally to this work.

  • Dr. J. Alexander Liddle

    Corresponding author
    1. Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA)
    • Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA)
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  • Dr. S. H. Ko and Dr. K. Du acknowledge support under the Cooperative Research Agreement between the University of Maryland and the National Institute of Standards and Technology Center for Nanoscale Science and Technology, award number 70NANB10H193, through the University of Maryland. The authors are grateful to Dr. H. Lezec and Dr. G. M. Gregg for helpful advice. The authors thank Dr. V. Szalai for helpful discussions and helping with the fluorimeter and Prof. A. Agrawal for comments. The authors also thank Dr. H. Yoon and Dr. J. Schumacher for helping with SEM imaging and Dr. T. Lam for TEM imaging.

Abstract

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Single-molecule GPS: The average photon count rate and lifetime of a quantum dot (Qdot) have been controlled by varying the geometrical configuration of Qdot–gold nanoparticle (AuNP) conjugates on DNA origami. With a 3D real-time single-molecule tracking system, which allows the DNA templates to be kept in their native state in solution, the influence of AuNPs on the Qdot lifetime is determined (see picture).

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