• Open Access

Embedding of Active Proteins and Living Cells in Redox-Sensitive Hydrogels and Nanogels through Enzymatic Cross-Linking

Authors

  • Smriti Singh,

    1. DWI e.V. and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Forckenbeckstrasse 50, 52056 Aachen (Germany)
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    • These authors contributed equally to this work.

  • Fuat Topuz,

    1. DWI e.V. and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Forckenbeckstrasse 50, 52056 Aachen (Germany)
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    • These authors contributed equally to this work.

  • Kathrin Hahn,

    1. Department and Chair of Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070 Würzburg (Germany)
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  • Dr. Krystyna Albrecht,

    1. Department and Chair of Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070 Würzburg (Germany)
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  • Prof. Jürgen Groll

    Corresponding author
    1. Department and Chair of Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070 Würzburg (Germany)
    • Department and Chair of Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070 Würzburg (Germany)
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  • This work was supported by the DFG (SPP 1259 “Intelligent Hydrogels”) and the EU (FP 6, project NanoBio-Pharmaceutics). F.T. thanks the Marie Curie Initial Training Network “Hierarchy” (project reference: 215851). Aplagen is acknowledged for peptide supply.

Abstract

original image

Horseradish peroxidase (HRP) can be used for the enzymatic cross-linking of thiol-functionalized polymers under mild conditions to form hydrogels and nanogels without the need for added H2O2. Cells can be embedded in the hydrogels and proteins can be entrapped and released from the nanogels. These gels are fully degradable under mild and cytocompatible reductive conditions.

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