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Self-Healing Microencapsulation of Biomacromolecules without Organic Solvents

Authors

  • Dr. Samuel E. Reinhold,

    1. Department of Pharmaceutical Sciences, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 (USA)
    2. Present address: Upsher-Smith Laboratories, Inc. 6701 Evenstad Drive, Maple Grove, MN 55369 (USA)
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    • These authors contributed equally to this research.

  • Dr. Kashappa-Goud H. Desai,

    1. Department of Pharmaceutical Sciences, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 (USA)
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    • These authors contributed equally to this research.

  • Dr. Li Zhang,

    1. Department of Pharmaceutical Sciences, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 (USA)
    2. Present address: Teva Parenteral Medicines, 19 Hughes, Irvine, CA 92618 (USA)
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  • Karl F. Olsen,

    1. Department of Pharmaceutical Sciences, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 (USA)
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  • Prof. Steven P. Schwendeman

    Corresponding author
    1. Department of Pharmaceutical Sciences, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 (USA)
    • Department of Pharmaceutical Sciences, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 (USA)
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  • This study was funded by NIH R01 HL 68345 and R21 EB 08873. We greatly appreciate the help of Shobha Churi and Dr. Manjunatha (JSS University, Mysore, India) in the procurement of the sample of tetanus toxoid. We are grateful to Dr. Rajesh Gupta, US Food and Drug Administration, for his assistance with ELISA and for providing the sample of equine tetanus antitoxin. We also thank Dr. Heather W. Collins, University of Pennsylvania School of Medicine, for conducting radioimmunoassays.

Abstract

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Capture and seal off all exits! Biomacromolecules are routinely microcapsulated in poly(lactic-co-glycolic acid) (PLGA) in multiple complex steps that are deleterious to the biomacromolecule. In contrast, PLGA encapsulation based on self-healing (see picture) shows high efficiency without protein damage and enables the stabilization and slow release of proteins.

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