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Keywords:

  • chemical ligation;
  • click chemistry;
  • proteins;
  • solid-phase synthesis;
  • synthetic methods
Thumbnail image of graphical abstract

Please release me: A new linker for the temporary tagging of peptides at their N-terminus after solid-phase elongation, and its potential for capture/release purification is demonstrated. This concept is extended to a remarkably efficient self-purifying N-to-C iterative triazole ligation strategy, which is applied to the synthesis of a polypeptide having 160 residues, in a high purity without the need for chromatographic purification (see picture; orange blocks: peptide segments).