These authors contributed equally to this work.
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Communication
Decrease in RNA Folding Cooperativity by Deliberate Population of Intermediates in RNA G-Quadruplexes†
Article first published online: 20 NOV 2012
DOI: 10.1002/anie.201206475
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Kwok, C. K., Sherlock, M. E. and Bevilacqua, P. C. (2013), Decrease in RNA Folding Cooperativity by Deliberate Population of Intermediates in RNA G-Quadruplexes . Angew. Chem. Int. Ed., 52: 683–686. doi: 10.1002/anie.201206475
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This study was supported by a Human Frontier Science Program (HFSP) Grant RGP0002/2009-C to P.C.B. and Penn State Summer Discovery Grant to M.E.S. We thank Prof. Sarah Assmann, Prof. Bratoljub Milosavljevic, and Dr. Melissa Mullen for helpful discussions.
- ‡
These authors contributed equally to this work.
Publication History
- Issue published online: 28 DEC 2012
- Article first published online: 20 NOV 2012
- Manuscript Revised: 9 SEP 2012
- Manuscript Received: 10 AUG 2012
Funded by
- Human Frontier Science Program (HFSP). Grant Number: RGP0002/2009-C
Keywords:
- fluorescence;
- folding cooperativity;
- G-quadruplexes;
- RNA;
- RNA structures
Keeping a broad (RNA) perspective: Conventional biochemical detection systems only have a 100-fold linear response range. The range of potassium concentrations detected by an RNA G-quadruplex sequence can be broadened by intentionally populating multiple intermediate folding states (see scheme). The folding of the RNA G-quadruplexes was monitored by both circular dichroism and intrinsic fluorescence spectroscopy.