Decrease in RNA Folding Cooperativity by Deliberate Population of Intermediates in RNA G-Quadruplexes


  • This study was supported by a Human Frontier Science Program (HFSP) Grant RGP0002/2009-C to P.C.B. and Penn State Summer Discovery Grant to M.E.S. We thank Prof. Sarah Assmann, Prof. Bratoljub Milosavljevic, and Dr. Melissa Mullen for helpful discussions.


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Keeping a broad (RNA) perspective: Conventional biochemical detection systems only have a 100-fold linear response range. The range of potassium concentrations detected by an RNA G-quadruplex sequence can be broadened by intentionally populating multiple intermediate folding states (see scheme). The folding of the RNA G-quadruplexes was monitored by both circular dichroism and intrinsic fluorescence spectroscopy.