These authors contributed equally to this work.
A Bioreducible Polymer for Efficient Delivery of Fas-Silencing siRNA into Stem Cell Spheroids and Enhanced Therapeutic Angiogenesis†
Article first published online: 16 OCT 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 47, pages 11899–11903, November 19, 2012
How to Cite
Shim, M. S., Bhang, S. H., Yoon, K., Choi, K. and Xia, Y. (2012), A Bioreducible Polymer for Efficient Delivery of Fas-Silencing siRNA into Stem Cell Spheroids and Enhanced Therapeutic Angiogenesis . Angew. Chem. Int. Ed., 51: 11899–11903. doi: 10.1002/anie.201206595
This work was supported in part by an NIH Director’s Pioneer Award (DP1 OD000798 to Y.X.) and NIH grants from the National Heart, Lung and Blood Institute (NHLBI, R01 HL55337 and R01 HL63736 to K.C.). We thank Dennis Oakley of the Bakewell Neuroimaging Core at Washington University School of Medicine for assistance with the confocal microscopy imaging.
- Issue published online: 14 NOV 2012
- Article first published online: 16 OCT 2012
- Manuscript Received: 15 AUG 2012
- NIH. Grant Number: DP1 OD000798
- National Heart, Lung and Blood Institute. Grant Numbers: R01 HL55337, R01 HL63736
Vol. 52, Issue 8, 2147, Article first published online: 13 FEB 2013
- intracellular delivery;
- stem cells;
- RNA interference
Larger is better: Fas-silencing siRNA can be delivered into the cytoplasm of human mesenchymal stem cells (hMSCs) by a bioreducible polymer (see picture). The enhanced anti-apoptotic activity of the Fas-silenced hMSCs means that they can be readily formulated as enlarged spheroids to significantly enhance angiogenic efficacy as compared to their smaller counterparts.