Monitoring β-Secretase Activity in Living Cells with a Membrane-Anchored FRET Probe


  • We thank the National Institutes of Health (grant GM084176), the Sloan Foundation, and the Camille and Henry Dreyfus Foundation for supporting this work. Special thanks to Sam Johnson and the Duke LMCF (grant 1S10RR027528-01) for their microscopy expertise.


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Turn it on! β-MAP is a sensitive FRET probe with specificity for monitoring the enzyme β-secretase (BACE), which is associated with Alzheimer's disease. After hydrolysis by the enzyme BACE, the probe fluoresces and thus allows real-time spatial and temporal assessment of enzymatic activity in living cells. β-MAP was used to confirm the cellular efficacy of a reported inhibitor without the need for mutated cell lines or antibodies.