Light-Addressable Capsules as Caged Compound Matrix for Controlled Triggering of Cytosolic Reactions

Authors

  • Markus Ochs,

    1. Fachbereich Physik and WZWM, Philipps Universität Marburg, Renthof 7, 35037 Marburg (Germany)
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    • These authors contributed equally to this work.

  • Dr. Susana Carregal-Romero,

    1. Fachbereich Physik and WZWM, Philipps Universität Marburg, Renthof 7, 35037 Marburg (Germany)
    2. Bionand. Severo Ochoa 35, 29590 Málaga (Spain)
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    • These authors contributed equally to this work.

  • Dr. Joanna Rejman,

    1. Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, Ghent (Belgium)
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  • Prof. Kevin Braeckmans,

    1. Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, Ghent (Belgium)
    2. Center for Nano and Biophotonics, Ghent University, Harelbekestraat 72, Ghent (Belgium)
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  • Prof. Stefaan C. De Smedt,

    1. Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, Ghent (Belgium)
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  • Prof. Wolfgang J. Parak

    Corresponding author
    1. Fachbereich Physik and WZWM, Philipps Universität Marburg, Renthof 7, 35037 Marburg (Germany)
    • Fachbereich Physik and WZWM, Philipps Universität Marburg, Renthof 7, 35037 Marburg (Germany)
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  • This work was supported by BMBF/ERANET (project Nanosyn) and the DFG (project PA794/11.1). We acknowledge technical discussions with Drs. Rafael Fernandez Chacón, Loretta del Mercato, Arnold Grünweller, Roland Hartmann, Pilar Riveral Gil and Gleb B. Sukhorukov.

Abstract

original image

Release me: Polyelectrolyte capsules with different cargo in their cavities and plasmonic and magnetic nanoparticles in their walls were synthesized. Enzymatic reactions were triggered inside cells by light-mediated opening of two individual capsules containing either an enzyme or its substrate, by using photothermal heating. Furthermore, this technique allows controlled release of mRNA from capsules, thereby resulting in synthesis of green fluorescent protein (GFP).

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