Exploring an Orbitrap Analyzer for the Characterization of Intact Antibodies by Native Mass Spectrometry

Authors

  • Sara Rosati,

    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH (The Netherlands) bioms.chem.uu.nl
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    • These authors contributed equally to this work

  • Dr. Rebecca J. Rose,

    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH (The Netherlands) bioms.chem.uu.nl
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    • These authors contributed equally to this work

  • Dr. Natalie J. Thompson,

    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH (The Netherlands) bioms.chem.uu.nl
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  • Dr. Esther van Duijn,

    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH (The Netherlands) bioms.chem.uu.nl
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  • Dr. Eugen Damoc,

    1. Thermo Fisher Scientific (Bremen), Hanna-Kunath Strasse 11, Bremen 28199 (Germany)
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  • Dr. Eduard Denisov,

    1. Thermo Fisher Scientific (Bremen), Hanna-Kunath Strasse 11, Bremen 28199 (Germany)
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  • Dr. Alexander Makarov,

    1. Thermo Fisher Scientific (Bremen), Hanna-Kunath Strasse 11, Bremen 28199 (Germany)
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  • Prof. Albert J. R. Heck

    Corresponding author
    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH (The Netherlands) bioms.chem.uu.nl
    • Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH (The Netherlands) bioms.chem.uu.nl
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  • This work was supported in part by STW (project 10805), the PRIME-XS project, Grant Agreement Number 262067, and by the PROSPECTS network (grant no. HEALTH-F4-2008-201648), both funded by the European Union Seventh Framework Program. The Netherlands Proteomics Centre, embedded in The Netherlands Genomics Initiative is acknowledged for funding. We would like to thank Merus and Genmab for their support and kind donation of materials.

Abstract

original image

Antibody profiling: Native mass spectrometry analysis of intact antibodies can be achieved with improved speed, sensitivity, and mass resolution by using a modified orbitrap instrument. Complex mixtures of monoclonal antibodies can be resolved and their glycan “fingerprints” can be profiled (see picture). Noncovalent interactions are maintained, thus allowing antibody–antigen binding to be measured.

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