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Absolute and Relative Quantification of Multiplex DNA Assays Based on an Elemental Labeling Strategy

Authors

  • Guojun Han,

    1. Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry Tsinghua University, Beijing 100084 (China)
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  • Sichun Zhang,

    1. Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry Tsinghua University, Beijing 100084 (China)
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  • Zhi Xing,

    1. Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry Tsinghua University, Beijing 100084 (China)
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  • Prof. Xinrong Zhang

    Corresponding author
    1. Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry Tsinghua University, Beijing 100084 (China)
    • Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry Tsinghua University, Beijing 100084 (China)
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  • This work was supported by the 973 Program (2013CB933800) and the National Natural Science Foundation of China (21027013; 21075075).

Abstract

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Elements and quantification: A nucleic acid assay has been developed, based on an elemental labeling strategy using magnetic microparticles (MMPs), which provides quantification of multiple DNA targets. Rare-earth elements, indium, and stable isotopes could be labeled with oligonucleotides serving as DNA probes. Quantitative analysis was then carried out using the designed systems (see picture) and elemental mass spectrometry.

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