Enhancement and Modulation of Enzymatic Activity through Higher-Order Structural Changes of Giant DNA–Protein Multibranch Conjugates


  • We thank G. Hallais and Dr. A. Gautier for experimental support and discussion. The research leading to these results received funding from the European Research Council (ERC) [European Community’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant agreement no. 258782] and from the Institut Universitaire de France (IUF).


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A giant DNA leap for enzyme-kind: Giant DNA–protein multibranch conjugates were shown to be composed of a single β-lactamase enzyme (green, see scheme) conjugated to up to four 48.5 kbp lambda phage DNA branches (blue lines). The conjugation of giant DNA induces a two- to fourfold enhancement of the enzymatic activity, which is modulated by changes in the higher-order DNA structure.